Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 7th October 1.1 Lab work 1.1.1 BioBrick K2039000 and K2039001 characterization 1.1.1.1 Culture of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594 Friday 7th October Lab work BioBrick K2039000 and K2039001 characterization Culture of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594 By Maxence The clones put on solid culture the 6th October were put on liquid cultures (LB + 30 µg/mL Cm ou Amp) grown at 37°C overnight: pPS16_018 (FKBP - GFP 10) clone 6 pPS16_019 (FRB - GFP 11) clone 4 pPS16_009 (GFP 1.9) clone 1 Furthermore, another strain containing the full GFP was cultures in the same conditions: PhB1040 strain 594 (lac-3350 galk2 galT22 2psL179 pGFP) (Amp resistance).
By Maxence
The clones put on solid culture the 6th October were put on liquid cultures (LB + 30 µg/mL Cm ou Amp) grown at 37°C overnight:
Furthermore, another strain containing the full GFP was cultures in the same conditions: PhB1040 strain 594 (lac-3350 galk2 galT22 2psL179 pGFP) (Amp resistance).
