Team:Austin UTexas/Demonstrate
| Species | Classification | Brand of Kombucha Isolated From |
|---|---|---|
| Staphylococcus warneri | Bacteria | GT’s Kombucha |
| Staphylococcus epidermidis | Bacteria | GT's Kombucha |
| Gluconobacter oxydans* | Bacteria | GT’s Kombucha |
| Lachancea fermentati* | Yeast | Buddha's Brew |
| Propionibacterium acnes | Bacteria | Buddha's Brew |
| Micrococcus luteus | Bacteria | Buddha's Brew |
| Bacillus pumilus | Bacteria | Buddha's Brew |
| Saccharomyces cerevisiae | Yeast | LIVE Soda Kombucha |
| Schizosaccharomyces pombe* | Yeast | LIVE Soda Kombucha |
(*Indicates a species that is considered vital to the production of kombucha)
pH Sensors
Possessing the ability to monitor the brewing process of kombucha without disturbing the microenvironment and using a very visible color reporter would allow for greater insights as to how the populations of organisms and pH may change due to competition amongst other bacteria and yeast in the beverage and SCOBY of the kombucha. The byproducts produced by the kombucha as it brews causes the tea to become more acidic, leading to our team searching for pH sensitive promoters, and for ways to implement these into kombucha.
Though an acidic sensor was what was required for our kombucha analysis, the identification of sensors in other areas of the pH spectrum were explored as well. Three sequences were identified, the CadC operon for the acidic range, CpxA-CpxR complex for the neutral range, and the P-atp2 promoter from the BioBrick Registry (BBa_K1675021) for the basic range. Each sequence was paired with a unique corresponding reporter sequence so that if each pH sensitive plasmid were in the same environment, the specific pH of the system could be seen. The reporters used were, BBa_E1010 for the CadC construct, BBa_K1033916 for the CpxA-CpxR complex, and BBa_K592009 for the P-atp2 promoter.
CadC
The CadC operon is a native pathway in E. coli, involved in the cadaverine synthesis pathway. The protein CadC protein on the operon is produced and activates segments downstream of the operon on the CadBA receptors. The CadC protein is pH sensitive to an external pH 5.5 and below, as well as lysine dependent. A point mutation on codon 265, in which argenine is converted to cystine, causes the CadC protein to become lysine independent (Dell, Neely, Olson, 1994).
Unfortunately, we have been unable to grow the modified CadC operon in E. coli suggesting some form of cell toxicity. Due to this apparent toxicity, no data regarding this mutant CadC could be collected. Alternative candidates are being explored for other pH sensors that sense in the acidic range.
CpxA-CpxR
P-atp2
The P-atp2 promoter, native to the bacterium Corynebacterium glutamicum is reportedly induced at pH 7, to pH 9 (XX_how to site another iGEM team?_XX). Utilizing the blue chromoprotein (BBa_K592009), a test was designed in which a plasmid containing the P-atp2 promoter with the blue chromoprotein was grown alongside an E. coli line that contained a plasmid with just the blue chromoprotein. We expected to see constant blue chromoprotein production in the control series (those that lacked P-atp2) and a visual increase in blue chromoprotein as the pH was raised from 6 to 9 in the cells that contained the P-atp2 construct. Next Steps and the GOX Sequences as Putative Promoters
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