Team:SDU-Denmark/Parts

Parts


Basic

BBa_K2018002

This BioBrick contain the coding region of Laterosporulin, a bacteriocin produced by Brevibacillus sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: B. subtilis , S. aureus, E. coli, P. aeruginosa,  and L. monocytogenes.

BBa_K2018003

This BioBrick contain the coding region of, Thuricin S, a bacteriocin produced by Bacillus thuringiensis. Thuricin S target a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.

BBa_K2018006

This BioBrick contain the coding region of Lacticin Q, a bacteriocin  produced by Lactococcus lactis QU5  and has shown bactericidal activity against Staphylococcus aureus [http://www.ncbi.nlm.nih.gov/pubmed/22538663]. It function by forming large toroidal pores by distributing membrane lipid organization.

BBa_K2018008

This BioBrick contain the coding region of Laterosporulin-Thuricin S, which is a hybrid bacteriocin with  Laterosporulin and Thuricin S we had designed.  

BBa_K2018009

This BioBrick contain the coding region of Lacticin Q-Lacticin Z, which is a hybrid bacteriocin with Lacticin Q and Lacticin Z we had designed.  

BBa_K2018018

This BioBrick contain the coding region of Pyocin S5, a bacteriocin produced by  a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death [http://www.sciencedirect.com/science/article/pii/S0014579310005120].

BBa_K2018024

p>This BioBrick contains the coding region of our biofused phasin a bacteriocin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.

BBa_K2018026

p>This BioBrick contains the entire DNA sequence prior to hemolysin B natively in Ralstonia eutropha. The last part of this biobrick is the native RBS for hemolysin B.