Collaborations
Cambridge JIC-team, England
The Cambrige JIC-team were genereating a chloroplast transformation toolkit and were interested in expressing a CRISPR/Cas9-system in the chloroplats of Chlamydomonas reinhardtii. The purpose of the project was to achieve homoplasmy quicker, in a matter of days rather than months since no selective plating would be required.
This has been an ongoing cooperation in the form of e-mail contact, skype meeting and weekly reports about the week that was. The exchange has included discussions, questions about the model organism, gibson assembly and other approaches, different protocols and other things that felt unclear.
USP/UNIFESP, Brazil
The Brazil team USP/UNIFESP also work with C. reinhardtii and wanted to try CRISPR/Cas9 with RNPs but encountered som difficulties when it turned out that another team worked on a similar project. The team needed a new approach which we helped them with. This was done by exchancing protocols and regular updates from both teams. Northwestern, USA Received protocols for several experiments and exchanged ideas to optimize protocols. The main collaboration was in the beginning of the period.
Collaborations via surveys
Surveys are a easy way to reach the public, LiU iGEM both answered and shared surveys from other teams in order to help them in their work.
Collaborations via surveys were with following teams:
-Team Chalmers, Sweden
-Team XMU, China
-Team Aachen, Germany
-LMU and TU Münich, Germany