Team:SDU-Denmark/Parts
Parts
This BioBrick contains the coding region of Phasin. Phasin binds to and reduces the size of PHA granules.
BioBrick encodes a short hemolysin tag. This tag can be attached to other proteins for secretion through the hemolysin pathway.
This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029).
This part is a functional BioBrick contain the coding region of Laterosporulin, , with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by Brevibacillus sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: B. subtilis , S. aureus, E. coli, P. aeruginosa, and L. monocytogenes.
This BioBrick contain the coding region of, Thuricin S, a bacteriocin produced by Bacillus thuringiensis. Thuricin S target a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.
This BioBrick contain the coding region of Lacticin Q, a bacteriocin produced by Lactococcus lactis QU5 and has shown bactericidal activity against Staphylococcus aureus [http://www.ncbi.nlm.nih.gov/pubmed/22538663]. It function by forming large toroidal pores by distributing membrane lipid organization.
This BioBrick contain the coding region of Laterosporulin-Thuricin S, which is a hybrid bacteriocin with Laterosporulin and Thuricin S we had designed.
This BioBrick contain the coding region of Lacticin Q-Lacticin Z, which is a hybrid bacteriocin with Lacticin Q and Lacticin Z we had designed.
This BioBrick contain the coding region of Pyocin S5, a bacteriocin produced by a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death [http://www.sciencedirect.com/science/article/pii/S0014579310005120].
This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.
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