Team:UT-Tokyo/Project
Introduction
The researches conducted so far have mostly been transforming Escherichia coli by changing external environments or introducing inductive factors. To the best of our knowledge, phenotypes of E. coli switch without any input from the exterior.
Until now, no study in iGEM competition has been done on changing autonomously phenotypes of E. coli before and after cell division.
Therefore, we have designed a system which automatically generates three phenotypes of E.coli every time a cell divides.
Specifically,in our system, if E. coli in which first generation of gfp is found divides, we cannot find gfp anymore but only rfp. If this second generation of E. coli divides then instead of rfp, cfp is expressed.
After another division in the fourth generation instead of cfp we find gfp again. In this system, the phenotype of E. coli differs from each other between the (3n)th, (3n+1)th, and (3n+2)th generation.
Figure
As we have created this genetic circuit，sigma factor, toehold switch and Pnrd promotors (link to the system) are chosen. In modeling we use them to simulate the system we made .(link to modeling) Modeling shows us whether the genetic circuit builded in thoughts is realizable.
In addition, after confirming the system runs on modeling, we ran an inspection on parts and devices in the experiment.
System
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Result
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Application
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