Team:UT-Tokyo/Model

iGEM UT-TOkyo 2016


Transcription and Translation

Subtitle

We found some parameter sets on which e-coli changes gene expression by a generation. In addition, we also found ones on which circuit establishes the loops stably regardless of small fluctuation (noise in experiment). Therefore, our gene circuit seems likely to work as designed.

Introduction

Method

Parameters character
sigma-factor A,B,C

$\sigma_A, \sigma_B, \sigma_C$

anti-sigma factor, A, B, C

$\sigma_{-A}, \sigma_{-B}, \sigma_{-C}$

mRNA of sigma factor A, B, C without toehold switch

$R_A, R_B, R_C$

mRNA of anti-sigma-factor A, B,C without toehold switch

$R_{-A}, R_{-B}, R_{-C}$

mRNA of sigma factor A, B, C with toehold switch

$R_{td, A}, R_{td, B}, R_{td, C}$

taRNA

$[\mathrm(taRNA)]$

IPTG

$[\mathrm(IPTG)]$

immature GFP, RFP, CFP

$[\mathrm(imGFP)], [\mathrm(imRFP)], [\mathrm(imCFP)]$

mature GFP, RFP, CFP

$[\mathrm(GFP)], [\mathrm(RFP)], [\mathrm(CFP)]$

mRNA of GFP, RFP, CFP

$R_{GFP}, R_{RFP}, R_{CFP}$


Stability Analysis

Abstract