Notebook
Week 28
cPCR program used:
Segment | Step | Temperature | Duration |
---|---|---|---|
1 | Initial denaturation | 98 °C | 2 min |
2 | 34 cycles | 98 °C | 10 sec |
72 °C | 15 sec | ||
72 °C | >15 sec | ||
3 | Final extension | 72 °C | 5 min |
4 | Keep the sample cold | 12 °C | HOLD |
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