Team:SDU-Denmark/Notebook
Notebook
Week 28
The brick containing the gene K2018011 was digested with PstI and XbaI overnight by using SOP0017_v01.
After digesting, the sample was ligated by following SOP0015_v01 with the standard iGEM plasmid, pSB1C3, overnight, and transformed into E.coli Top10.
cPCR program used:
| Segment | Step | Temperature | Duration |
|---|---|---|---|
| 1 | Initial denaturation | 98 °C | 2 min |
| 2 | 34 cycles | 98 °C | 10 sec |
| 72 °C | 15 sec | ||
| 72 °C | >15 sec | ||
| 3 | Final extension | 72 °C | 5 min |
| 4 | Keep the sample cold | 12 °C | HOLD |
After a colony PCR, SOP0021_v01, was run and verified on a gel, the following result was given: Our part is 259 bp and primer overhangs are 150 X 2 bp, which is, in total, 559 bp: A gel electrophoresis from cPCR showed a band around 600 bp, so we assumed that we had the correct part inserted into the iGEM standard plasmid.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
