Team:Austin UTexas/Demonstrate
Kombucha Strains
Key Achievements:
- Successfully isolated microbes from various samples of kombucha
- Identified strains of bacteria and yeast using rRNA gene sequencing
- Characterized each of the isolated microbes to facilitate further experimentation
Recapitulation
- Kombucha is not possible without the microbe Ga. hansenii.
- Multiple yeast are necessary, and while, our essential yeast were both Fermentati, these were different species strains.
- With the proper essential microbes, kombucha can be put back together from individual microbes.
Key Achievements:
Conjugation
Key Achievements
- Nothing
Ethanol
Key Achievements
- Identified genes involved in the metabolism of ethanol to acetic acid in the bacterium Ga. hansenii
- Designed Golden Gate parts for the assembly of these genes into a functional construct
- Used a bromothymol blue assay to compare changes in pH resulting from fermentation in multiple strains of Lachancea fermentati isolated from our kombucha
pH Sensors
Key Achievements
- Successfully created a neutral pH sensor.
- Characterized the Biobrick P-atp2.
- Identified a potential pH sensor in Gluconobacter oxydans.
