Team:Alverno CA/Proof

Alverno iGEM 2016

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Proof

Working Model of Software - Python Program (Code) for Analyzing Plate Reader Data *The plate reader we use is the Perkin Elmer Victor X3 2030 Multilabel Reader. *The results will show up as graphs. import matplotlib.pyplot as plt import pandas as pd import seaborn as sns #The name of the file you plan to use goes in the ‘’ for filename the line below. filename = '' df = pd.read_csv(filename) df = df.dropna() print df.head() # data = df[df['Well']=='A03'] # data = df[df['Repeat'] > 115] #The two lines above may be used if you want to test some wells that only satisfy specific conditions, for example Well A01, and all the repeat above 115. data = df[df['Well'].isin(['H06','H07','H08','H09'])] #The line above is used to test a collection of wells by their well name. You can add the names of the wells into []. #The following are the different wells on the plate we used, you may use something different #'A01','A02','A03','A04','A05','A06','A07','A08','A09','A10','A11','A12' #'B01','B02','B03','B04','B05','B06','B07','B08','B09','B10','B11','B12' #'C01','C02','C03','C04','C05','C06','C07','C08','C09','C10','C11','C12' #'D01','D02','D03','D04','D05','D06','D07','D08','D09','D10','D11','D12' #'E01','E02','E03','E04','E05','E06','E07','E08','E09','E10','E11','E12' #'F01','F02','F03','F04','F05','F06','F07','F08','F09','F10','F11','F12' #'G01','G02','G03','G04','G05','G06','G07','G08','G09','G10','G11','G12' #'H01','H02','H03','H04','H05','H06','H07','H08','H09','H10','H11','H12' sns.tsplot(data=data, time="Time RFP (seconds)", value="Normalized RFP", condition="Well", err_style="unit_traces", unit="Plate") plt.title("RFP Fluorescence") plt.ylabel("RFP(AFU)") plt.show() sns.tsplot(data=data, time="Time GFP (seconds)", value="Normalized GFP", condition="Well", err_style="unit_traces", unit="Well") plt.title("GFP Fluorescence") plt.ylabel("GFP(AFU)") plt.show() sns.tsplot(data=data, time="Time Abs (seconds)", value="Absorbance @ 600 (A)", condition="Well", err_style="unit_traces", unit="Well") plt.title("Absorbance") plt.ylabel("Absorbance(AAU)") plt.show() BioBrick Assembly: Plasmid with pSB1C3 Backbone ??