Team:Alverno CA/Collaborations

Collaborations

TX-TL is a cell-free, in vitro, transcription/translation system that allows for inexpensive and rapid prototyping of biological circuits. This is desirable as the usual method of prototyping and debugging circuits requires DNA parts to be cloned into cells, which can take a long time. With TX-TL, once all the DNA parts have been obtained, the circuit can be tested immediately, and so several circuit iterations can be tested in the time it takes to successfully clone even one circuit iteration into cells. Caltech was generous enough to lend us a plate reader and through this, we were able to use this method as a way to get results.

We offered to TX-TL William and Mary’s Team’s plasmids in order to see how their results would vary with this different construct. William and Mary’s Team gave us suggestions and also offered us some plasmid and backbone which is iGEM standard plasmid.

Below is a graph of our results