Team:Newcastle/Proof/VariableResistor

Results

Growth in Varying ZnSO₄ Concentrations (Minimum Inhibitory Concentration):</i>

In this experiment, we used 6mM of zinc sulphate (ZnSO₄) and carried out a dilution of half for each column of a 96 well plate to try and identify the minimum inhibitory concentration for the <i>E. coli</i> to grow in. Cells were again placed in the pSB1C3 backbone and fresh cultures were grown at 37°C overnight. The cells were diluted down to a starting optical density of around 0.05 at 600nm using LB broth with chloramphenicol. Then, 100mu;l of cells were placed in the wells with the corresponding ZnSO₄ concentration which was diluted using LB with chloramphenicol. This experiment was left for 16 hours to give the <i>E. coli</i> time to grow and replicate, see figure 1.