Team:Newcastle/Proof/VariableResistor

Results

Growth in Varying ZnSO₄ Concentrations (Minimum Inhibitory Concentration):

In this experiment, we used 6mM of zinc sulphate (ZnSO₄) and carried out a dilution of half for each column of a 96 well plate to try and identify the minimum inhibitory concentration for the E. coli to grow in. Cells were again placed in the pSB1C3 backbone and fresh cultures were grown at 37°C overnight. The cells were diluted down to a starting optical density of around 0.05 at 600nm using LB broth with chloramphenicol. Then, 100μl of cells were placed in the wells with the corresponding ZnSO₄ concentration which was diluted using LB with chloramphenicol. This experiment was left for 16 hours to give the E. coli time to grow and replicate, see figure 1.