Team:Technion Israel/Description
Chemotaxis
Chemotaxis is the movement of an organism in response to an external chemical stimulus.
Many single-cell
and multicellular organisms use chemotaxis to navigate their immediate environment.
The E. coli chemotaxis system is considered a model system that illustrates some of the core principles
of chemotactic movement (1). It allows the cell to sense and quickly respond to nearby nutrients
– attractants, and dangerous chemicals – repellents.
A detailed explanation on the chemotaxis system and the intercellular processes involved can be
found here.
Fig. 1: Chemotaxis concept (1).
Chemoreceptors – A bacterial sensor system
Bacterial chemotaxis is mediated by chemoreceptors. The purpose of these membrane proteins is to bind a certain
chemoeffector,
and transduce the signal to the downstream proteins.
The specificity of a chemoreceptor is determined by its ligand binding domain – the transmembrane region
of the receptor. We have chosen this domain as the focus of our project.
Fig. 2: Chemoreceptor structure illustration.
S.Tar – In control of chemotaxis
Project Super Tar - S.Tar in short, is designed to be a novel platform for controlled chemotaxis.
The base of our project is the E. coli Tar chemoreceptor or more specifically, its ligand binding
domain (LBD).
We started by improving the Tar chemoreceptor BioBrick found in the iGEM
kit (designed by team Goettingen 2014).
Moreover, we show that by mutating the native Tar LBD (2) or by interchanging it with that of other
receptors (3), the E. coli chemotaxis system can be programmed to respond to completely new ligands.
Fig. 1: chemoreceptor structure illustration. (source@#$%^&*)
To demonstrate the potential of our system, we followed two approaches:
1. We replaced the Tar LBD with that of the PctA (link to the chimeras page) chemoreceptor and demonstrated (link to PctA results)
a chemotactic response to its repellent – TCE, a substance which the native Tar cannot recognize.
2. We used computational biology to redesign the native Tar receptor (link to Rosetta page)
to bind Histamine instead of its original ligand- Aspartate. We demonstrated a chemotactic response from the mutated receptor (link to histamine results).
Using S.Tar, scientists will be able to control the movement of bacteria and direct them towards or away
from a target material. This system can potentially have vast applications in bioremediation and, as we show in our
project, detection.
FlashLab – A S.Tar detector
As a further application of our system we designed an easy-to-use detection system which utilizes the high sensitivity of the chemotactic response. FlashLab (link to flashlab page) is a simple and cheap platform for the detection of any chemoeffector, using S.Tar bacteria.
Fig. 1: chemoreceptor structure illustration. (source@#$%^&*)
1. Bi, S. and Lai, L., 2015. Bacterial chemoreceptors and chemoeffectors. Cellular and Molecular Life Sciences, 72(4), pp.691-708.
2. Moretti, R., Bender, B.J., Allison, B. and Meiler, J., 2016. Rosetta and the Design of Ligand Binding Sites. Computational Design
of Ligand Binding Proteins, pp.47-62.
3. Reyes‐Darias, J.A., Yang, Y., Sourjik, V. and Krell, T., 2015. Correlation between signal input and output in PctA and PctB amino
acid chemoreceptor of Pseudomonas aeruginosa. Molecular microbiology, 96(3), pp.513-525.
