Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 29th June 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Culture of clones with gBlocks 1.1.2 BioBrick characterization 1.1.2.1 BL21 electro-competent cells preparation Wednesday 29th June Lab work Visualization Culture of clones with gBlocks By Lea and Marion We got 6 clones of each construction in 3,5ml LB (50 µg/ml Amp). Incubation overnight at 37°C, 180rpm. BioBrick characterization BL21 electro-competent cells preparation By Charlene We added 200µL of BL21 pre-culture in 15mL of LB. It incubated 4h at 37°C. When abs600nm=0,68 cells have been centrifuged 10 minutes at 4000rpm. We did 2 washes with addition of 10mL glycerol (10%) followed by 10 minutes of centrifugation at 4000rpm. We putted cells in 200 µL glycerol.
By Lea and Marion
We got 6 clones of each construction in 3,5ml LB (50 µg/ml Amp). Incubation overnight at 37°C, 180rpm.
By Charlene
We added 200µL of BL21 pre-culture in 15mL of LB. It incubated 4h at 37°C. When abs600nm=0,68 cells have been centrifuged 10 minutes at 4000rpm. We did 2 washes with addition of 10mL glycerol (10%) followed by 10 minutes of centrifugation at 4000rpm. We putted cells in 200 µL glycerol.
