Team:Ionis Paris/Parts
The biosensor is our favorite part because it is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.
As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our: Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria. We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly. We organized and attended to a lot of events during the year. Whether it is iGEMers Meetups, professional events, or even sports events that will help us raise money, our team was very active ! We loved participating to all of these events, and definitely keep some very good memories from them. As we are quite a recent team in the Paris region, we tried to meet the other iGEM Teals and collaborate with them as much as we could. We had a lot of collaborations with different teams, mainly from the Paris area, the most important one being the organization of our biggest event, the European Experience.Favorite Part: our biosensor
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023001
BB123
Biosensor device
Measurement
C.Chenebault, C.Soucies, B.Piot
3156 bp
Biosensor parts list
Collaborations
