Team:IIT-Madras
Welcome to IIT Madras's wiki for iGEM 2016!
When we started to ideate for iGEM 2016, our goals were to tackle two of the fundamental issues being faced in synthetic biology today - estimating modularity of RBSs and predicting the variations in the protein expression levels and designing an efficient measurement device. We have also designed efficient ribo switches and been using them for several potential applications e.g. inhibition of HGT etc. Our final project will consist of 3 modules - RIBOS riboregulatory switches, efficient measurement device, and characterizing widely used RBSs for their modularity.
RIBOS(RNA Inducible Boolean like Output Switches) is a regulatory system in which presence or absence of mRNA molecules of one gene can modulate the expression of other genes. RIBOS devices are triggered by RNA sequences and give output in the form of activation or repression of translation of specific RNA sequences. We have designed RIBOS for scalability, modularity, orthogonality and composability. We wish to characterize RIBOS for tunability and highly precise outputs. It can be game changing in the field of synthetic biology, it's applications range from detection and quantification of mRNA molecules to the design of extensive independent and modular genetic circuits using forward engineering.
