Team:Ionis Paris/Parts
The biosensor is our favorite part because it is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.
Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria. We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.Favorite Part: our biosensor
Part Name
Nickname
Type
Designer
Lengh
BBa_K2023001
Biosensor device
BB123
Measurement
C.Chenebault, C.Soucies, B.Piot
3156 bp
Biosensor parts list
Part Name
Nickname
Type
Designer
Lengh
BBa_K2023004
Pr-RBS
BB1
Composite
C.Chenebault
448 bp
enlever titre
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023005
XylR
BB2
Coding
C.Soucies
1704 bp
A enlever
Part Name
Nickname
Type
Designer
Lengh
BBa_K2023006
Term-Pu-RBS-Gluc-term
BB3
Composite
B.Piot
1330 bp
Biosensor parts list
Part Name
Nickname
Type
Designer
Lengh
BBa_K2023002
XylR coding device
BBA
Regulatory
C.Chenebault
2261 bp
enlever titre
Part Name
Nickname
Type
Designer
Lengh
BBa_K2023003
Gluc coding device with Pu
BBB
Reporter
C.Soucies
1028 bp
Parts for CelloCad
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023014
GFP coding device with Pr
C2
Device
V.Plait
1314
enlever titre
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023015
XylR coding device-GFP coding device with Pu
C3
Measurement
C.Soucies
3391 bp
A virer
Part Name
Nickname
Type
Designer
Lengh
BBa_Bba_K2023017
Pu-RBS
C4
Composite
C.Chenebault
358 bp
enlever titre
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023016
GFP coding device with Pu
C45
Reporter
C.Soucies
1224 bp
Biosensor parts list
As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.
Learn more about our work with CelloCadB
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:
Parts & Characterization Proof of ConceptOur project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.
Learn more about our HardwareWe investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.
Learn more about the Entrepreunarial aspects of our project