Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 30th June 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Extraction of the plasmids containing gBlocks 1.1.1.2 Digestion of the plasmids containing gBlocks Thursday 30th June Lab work Visualization Extraction of the plasmids containing gBlocks By Alice and Lea DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing. Digestion of the plasmids containing gBlocks After extraction, plasmids were digested with EcoRI and HindIII - Component Volume (µL) Plasmids 3 Red buffer 10x 2 Water 14 EcoRI enzyme 0.5 HindIII enzyme 0.5 The mix was incubated for 1 hour at 37°C and then stored at -20°C over the night.
By Alice and Lea
DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.
After extraction, plasmids were digested with EcoRI and HindIII
The mix was incubated for 1 hour at 37°C and then stored at -20°C over the night.
