Team:Freiburg/Results

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Overview
image/svg+xml Inflamed area of colon epithelium - in case of ulcerative colitis parts of colon and rectum are inflamed because of an autoimmune reaction. zellen Carcinoembryonic antigen (CEA) - in the inflamed area the expression of CEA is higher than in the healthy tissue. This makes them a perfect target for our project. CEA Carrier - Using the endospores of Bacillus subtilis as a carrier we receive a tool with multivalency. This increases the specificity of binding to diseased tissue in comparison to healthy tissue where CEA is expressed in low concentration. To gain multivalency a carrier is used. Spore Drug Delivery - Glutathione-S-Transferase is an enzyme that is responsible for several detoxification reactions in the liver. We use it locally to convert an inactive prodrug into the active drug. gst The Binding - The Nanobody, a single domain antibody, binds to the CEA with a very high specificity. With its easy production and high stability it makes the perfect binding tool for our project. nb Glutathione S-Transferase forms the reaction from the inactive prodrug azathioprine to the active 6-Mercaptopurine, which replaces adenine and guanine in the DNA and finally leads to the apoptosis of the cell. AZA
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Posted by: iGEM Freiburg

Nanocillus - 'cause spore is more!