Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 1st July 1.1 Lab Work 1.1.1 Biobrick characterization 1.1.1.1 Buffer preparations 1.1.2 Visualization 1.1.2.1 Migration of plasmids containing gBlocks digestion Tuesday 1st July Lab Work Biobrick characterization Buffer preparations by Charlene and Caroline Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL) Na2HPO4 – 3,125g NaH2PO4.H2O – 1,38g KCl – 0,185g MgSO4 – 0,04g β-mercaptoethanol – 0,893mL Trisphosphate pH 7,8 1M – 6,25mL MgCl2 2M – 1mL DTT 1M -0,25mL EDTA 0,5M – 0,5mL BSA – 0,25g Glycerol 60% - 62,5mL Triton 10% (100x) – 25mL Na2CO3 1M – 0,78g Visualization Migration of plasmids containing gBlocks digestion Products of the digestion made the 30/06/16 are migrated on a gel (0.8% agarose) during 30 min, 100 V. Component Volume (µL) Digested DNA 10 Loading buffer 2
by Charlene and Caroline
Na2HPO4 – 3,125g
NaH2PO4.H2O – 1,38g
KCl – 0,185g
MgSO4 – 0,04g
β-mercaptoethanol – 0,893mL
Trisphosphate pH 7,8 1M – 6,25mL
MgCl2 2M – 1mL
DTT 1M -0,25mL
EDTA 0,5M – 0,5mL
BSA – 0,25g
Glycerol 60% - 62,5mL
Triton 10% (100x) – 25mL
Na2CO3 1M – 0,78g
Products of the digestion made the 30/06/16 are migrated on a gel (0.8% agarose) during 30 min, 100 V.
