Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 23rd June 1.1 Lab work 1.1.1 Competent cells 1.1.2 Interlab study 1.1.2.1 Transformation results 1.1.2.2 Transformed cell cultures Thursday 23rd June Lab work Competent cells by Caroline and Lea A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed. Interlab study Transformation results By Caroline, Lea and Marion Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely. Transformed cell cultures By Lea All colonies that were obtained after transformation were put into culture. One colony was taken into 3,5mL LB medium + Cm at 30µg/mL.
by Caroline and Lea
A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed.
By Caroline, Lea and Marion
Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.
By Lea
All colonies that were obtained after transformation were put into culture. One colony was taken into 3,5mL LB medium + Cm at 30µg/mL.
