Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 5th July 1.1 Lab work 1.1.1 Visualization 1.1.1.1 4,1 and 2,2 transformation Tuesday 5th July Lab work Visualization 4,1 and 2,2 transformation The 04/06/2016 transformations show white colony growth for G-blocks 4.2 clone 3 and GFP1-9 (clone 1), but blue colonies only for 4.1 clone 3. Thus this last G-block 4.1 clone 3 was transformed again following the same protocol as the 21/06/2016 with : 50μg of competent DH5α cells 4.1 ligation product in 5μL of the plasmid puc19 4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis) 6 clones of each of the other transformations 1.1, 1.2, 2.1, 3.1 and 3.2 were placed on culture in 4mL of LB added to Ampicillin (50 Protocol: -
The 04/06/2016 transformations show white colony growth for G-blocks 4.2 clone 3 and GFP1-9 (clone 1), but blue colonies only for 4.1 clone 3.
Thus this last G-block 4.1 clone 3 was transformed again following the same protocol as the 21/06/2016 with : 50μg of competent DH5α cells 4.1 ligation product in 5μL of the plasmid puc19 4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)
6 clones of each of the other transformations 1.1, 1.2, 2.1, 3.1 and 3.2 were placed on culture in 4mL of LB added to Ampicillin (50 Protocol: -
