Team:SDU-Denmark/Perspectives
Perspectives
Overview
Bacteriocin results
- Purification of the bacteriocins by the IMPACT method
- Determination of bacteriocin protein concentration using Bradford Protein Assay
- Inhibition of growth of S. aureus MRSA strains and P. aeruginosa detected by MIC
- Synergistic effect of hybrid bacteriocins detected by MIC
Constructing silk fibers
- Successfull insertion of the MaSp1 and MaSp2 gene fragments into pSB1C3
- Successful ligation test
- Proof the concept of preparing silk by the ICA method for one monomer of MaSp1 and MaSp2
- Missed ligation of a full construct
Production optimization of plastic producing cells
Bacteriocin purification (Top)
Figure 1 shows results of a cPCR originating from respective colonies from transformation of K2018011/pTXB1/E.coli Top10 (ThuricinS), K2018012/pTXB1/E.coli Top10 (LacticinQ) and K2018014/pTXB1/E.coli Top10 (Laterusporulin-ThuricinS). From right; Well 1-3 + 5-7 + 13-14= K2018011, Well 4+8 = K2018014, Well 9-12 = K2018012. The expected length of ThuricinS is 304 bp. GeneRulerTM 100 bp DNA ladder were used ThermoFisher scientific. .
