Perspectives
Overview
Bacteriocin results
- Purification of the bacteriocins by the IMPACT method
- Determination of bacteriocin protein concentration using Bradford Protein Assay
- Inhibition of growth of S. aureus MRSA strains and P. aeruginosa detected by MIC
- Synergistic effect of hybrid bacteriocins detected by MIC
Constructing silk fibers
- Successfull insertion of the MaSp1 and MaSp2 gene fragments into pSB1C3
- Successful ligation test
- Proof the concept of preparing silk by the ICA method for one monomer of MaSp1 and MaSp2
- Missed ligation of a full construct