Member’s Attributions
All the data presented on this wiki was measured, collected, and analyzed by the team members. All the plasmids directly involved in the data presented on this wiki were constructed by the team members. The idea conceiving, content planning, execution, and result analysis of human practice were all done by the team members. The following is the detailed attribution:
LI Cheng mainly focused the overall operating of the team, meanwhile he took a part in measurement of protein function. He was involved in team interaction and academic communication.
YANG Xiaoyu was responsible for protein purification and some evaluation experiments for the system.
BAI Ke was in charge of all experiments related to secretion, including plasmid construction, gene expression and secretory protein examination; He is also one of the speakers who give presentation.
DONG Yimingwas responsible for doing experiment including the building of secretion module, parts and the interlab.
LI Yuexuan worked as the financial manager of the team. She was also responsible for plasmid construction and submission.
YANG Changru was mainly responsible for the test experiment (crosslinking) of protein and modeling, as well as designing some genes, doing some quantitative experiments of uranyl absorption and secretion with the cooperation of teammates.
ZHAO Shijun devoted himself to molecular cloning and part construction.
LV Nayun has taken part in the protein extraction and the analysis of the questionnaire results.
WANG Dingyu was responsible for protein purification and some art design
LI Jiamian was the art designer and photographer of our team and he participated in wiki building and PowerPoint design.
WEI Jingyi was responsible for plasmid construction for Secretion Part.
WANG Yuqing was the lab manager and in charge of wiki building, she also participated in human practices, art design and part of the experimental work.
TENG Huaiyuan was in charge of modeling and software design. I studied Flory J.Paul's theory in polymer chemistry and furthermore established the model of molecular weight distribution in our case.
NING Xin was mainly responsible for the molecular cloning of the secretion system ，while participating in the measurement of proteins's functions as well.
CHANG Xuyao was in charge of plasmid construction of the Secretion Part.
Acknowledgement
We'd like to thank all those who have helped us over the summer, without whose sincere support The Uranium Reaper Project goals would have not been achieved.
General support
Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian and other teachers helped us during our brainstorming and gave us useful suggestions.
Prof. OUYANG Qi and Prof. LIU Chunli kindly provided the laboratory to us for experiments.
ZHANG Yihao gave us meticulous guidance and help.
Materials support
Dr. ZHANG Wenbing provided us with the plasmid of 3B(Tri-SpyCatcher within 15X linker).
Prof. ZHOU Lu provided us with Uranyl-binding Protein, also called SUP.
Prof. LIU Chunli allowed us to use his lab to do experiments on uranyl ion. The uranyl nitrate solution and Arsenazo III was provided by Prof. LIU.
Prof. LOU Chunbo provided us with the strain B.subtilis and mSA, heavy metal ions binding proteins: lead-binding protein, mercury-binding protein and cadmium-binding protein.
Dr. DU Pei provided the beads to us and gave us experimental guidance.
BIT-China provided us with the important Interlab kit.
Experiment equipment support
Prof. OUYANG Qi lent the Akta Pure to us for all proteins purification.
Prof. CHANG Zengyi and Dr. WANG Qingsong provided us with Scanner and helped with the setup and scan of gels.
Prof. LIU Chunli provided us with Geiger counter to test the radiation level in and around our lab.
Core Facilities, School of Life Sciences, Peking University,assisted us with ITC.
The Testing center of College of Environmental Sciences and Engineering, PKU provided the Aurora M90 mass spectrograph.
Theoretical guidance
ZHANG Yihao and WANG Yu, the wiki designers of Peking iGEM 2015,gave us many suggestions about wiki building.
Dr. YU Daqi of Prof. OUYANG Qi’s lab helped us a lot in experimental operating and data analysis.
Prof. ZHANG Wenbing, Prof. LOU Chunbo and Dr. YU Daqi kindly helped our team with modeling of the project.
Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian and ZHANG Yihao, the instructors, coached us for the presentation.
LI Ying and ZHANG Xueping provided us with kind suggestions of our presentation.
Prof. LAI Luhua, Prof. TANG Chao, Prof. CHEN Xing, Prof. CHEN Peng and Prof. LI Chenjian participated in our presentation rehearsal and gave suggestions for revisions.
Yiwen Expert Text Solutions helps our team correct the content of our wiki.
NWPU offered us helpful suggestions of PowerPoint.
Synthetic biology course
From March to June, an introductory course of synthetic biology was hold by College of Life Sciences, Peking University.During the course, the teachers, also the instructors gave us kind guidance and judged our project design. In late June, we started in the lab to start our project.
