Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 21st June 1.1 Lab work 1.1.1 Competent cells preparation 1.1.2 Interlab study 1.1.2.1 Transformation (cf. protocole Tuesday 21st June Lab work Competent cells preparation By Lea, Caroline and Marion Culture OD was measured at 600nm (OD = 3.4). 3.4mL were put into 250mL of LB medium and incubated at 20°C and 180rpm overnight. Interlab study Transformation (cf. protocole By Lea, Caroline and Marion 50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. Each transformation condition was displayed into petri dish in duplicate and then incubated at 37°C overnight.
By Lea, Caroline and Marion
Culture OD was measured at 600nm (OD = 3.4). 3.4mL were put into 250mL of LB medium and incubated at 20°C and 180rpm overnight.
50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. Each transformation condition was displayed into petri dish in duplicate and then incubated at 37°C overnight.
