Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Monday 11th July 1.1 Lab work 1.1.1 Biobrick characterization 1.1.1.1 Electrocompetents and transformation by electroporation of BL21 Monday 11th July Lab work Biobrick characterization Electrocompetents and transformation by electroporation of BL21 By Charlène BL21 cells were grown up to OD600nm=7.14. They were then made competent and transformed with K1372001 extracted from clone 2 and pcl_TAA, pcl_TAG or pcl_Tq to created the following strains: BL21|K1372001+pcl_TAA BL21|K1372001+pcl_TAG BL21|K1372001+pcl_Tq
By Charlène
BL21 cells were grown up to OD600nm=7.14. They were then made competent and transformed with K1372001 extracted from clone 2 and pcl_TAA, pcl_TAG or pcl_Tq to created the following strains:
