Team:Newcastle/Notebook/Lab/Lightbulb/Week 15

September 26th 2016

Today we entered the lab full of anticipation to see the results from the plate reader set up the previous week. Unfortunately, the results were not as we hoped. Many of the wells had AGAIN saturated the detection limits of the plate reader. After talking to our advisers, we decided a more appropriate negative control was required so as a result from this point forward we used BL21 wild type E.coli cells; the new plate layout is as follows. We inoculated fresh liquid LB broth for use with the plate reader and testing the battery constructs: