Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 23rd June 1.1 Lab work 1.1.1 Heat-shock competent cells 1.1.2 Interlab study 1.1.2.1 Transformation results 1.1.2.2 Transformed cell cultures Thursday 23rd June Lab work Heat-shock competent cells By Caroline and Lea The cells competency was checked by transforming cells as usual with two control plasmids: the Interlab study negative control pSB1C3 blue Interlab study Transformation results By Caroline, Lea and Marion Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely. Transformed cell cultures By Lea All colonies that were obtained after transformation were put into culture. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.
By Caroline and Lea
The cells competency was checked by transforming cells as usual with two control plasmids:
By Caroline, Lea and Marion
Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely.
By Lea
All colonies that were obtained after transformation were put into culture. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.
