Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 13th July 1.1 Lab work 1.1.1 Getting DNA closer 1.1.1.1 1.1.1.2 1.1.2 Biobrick characterization 1.1.2.1 BL21 electrocompetent cells preparation and transformation 1.1.3 Visualization 1.1.3.1 Wednesday 13th July Lab work Getting DNA closer Glycerol stocks for DH5α tra,sformed with DS-NMcas, DS-SPcasN-, DS-ST1casN- and DS-TDcasN- By Laetitia 8 stocks were made: 2 clones (Cl 1 and Cl 2) by cas: Cl1 and Cl2 of DS-NMcas Cl1 and Cl2 of DS-SPcasN- Cl1 and Cl2 of DS-ST1casN- Cl1 and Cl2 of DS-TDcasN- For 1 glycerol stock: 1mL of liquid culture 500 μL of glycerol 60% Biobrick characterization BL21 electrocompetent cells preparation and transformation By Mathilde and Charlène We did an electro-transformationof BL21 with : pcl_TAA + K1372001 (time constant equal to 5.9ms) pcl_TAG + K1372001 (time constant equal to 6 ms) pcl_Tq + K1372001 (time constant equal to 6 ms) Cells were displayed on LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. For each condition, we made a dish with 50µL of cells and another with 500µL of cells. Visualization
Glycerol stocks for DH5α tra,sformed with DS-NMcas, DS-SPcasN-, DS-ST1casN- and DS-TDcasN-
By Laetitia
8 stocks were made: 2 clones (Cl 1 and Cl 2) by cas:
For 1 glycerol stock: 1mL of liquid culture 500 μL of glycerol 60%
By Mathilde and Charlène
We did an electro-transformationof BL21 with :
Cells were displayed on LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. For each condition, we made a dish with 50µL of cells and another with 500µL of cells.
