Team:Austin UTexas/Protocols

HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure

5 g of Yeast Extract
5 g of Peptone
2.7 g of Sodium Phosphate, dibasic anhydrous (Na₂HPO₄)
1.5 g of Citric Acid
16 g of Agar (if making solid media)
Add distilled water to reach final volume
Autoclave the solution
50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS

YPD Media
Introduction
This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
Procedure

10 g of Yeast Extract
20 g of Peptone
16 g of Agar (if making solid media)
Add distilled water to reach final volume
Autoclave the solution
50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS

PBS (Phosphate Buffered Saline) Solution
Introduction
This is the recipe to make 500 mL of PBS which is used in a variety of procedures.
Procedure 1. Add 400 mL to an empty bottle 2. Add and dissolve each of the following compounds in the water:

4.0 g of Sodium Chloride (NaCl)

0.1 g of Potassium Chloride (KCl)

0.72 g of Sodium Phosphate, dibasic anhydrous (Na₂HPO₄)

0.12 g of Potassium Phosphate, monobasic (KH₂PO₄) </li)