Team:Austin UTexas/Protocols


HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure

  1. Add the following coumpound
    • 5 g of Yeast Extract
    • 5 g of Peptone
    • 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
    • 1.5 g of Citric Acid
    • 16 g of Agar (if making solid media)
  2. Add distilled water to reach final volume
  3. Autoclave the solution
  4. Add 50 mL of Sterile 40% (w/v) glucose after the solution has been autoclaved

DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS

YPD Media
Introduction
This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
Procedure

  1. Add the following compounds
    • 10 g of Yeast Extract
    • 20 g of Peptone
    • 16 g of Agar (if making solid media)
  2. Add distilled water to reach final volume of 1 L
  3. Autoclave the solution
  4. Add 50 mL of Sterile 40% (w/v) glucose after the solution is autoclaved

DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS

PBS (Phosphate Buffered Saline) Solution
Introduction
This is the recipe to make 500 mL of PBS which is used in a variety of procedures.
Procedure

  1. Add 400 mL to an empty bottle
  2. Add and dissolve each of the following compounds in the water:
    • 4.0 g of Sodium Chloride (NaCl)
    • 0.1 g of Potassium Chloride (KCl)
    • 0.72 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
    • 0.12 g of Potassium Phosphate, monobasic (KH2PO4)
  3. Autoclave the solution



4x Sucrose Solution
Introduction
This is the recipe to make 472.5 mL of 4x Sucrose Solution
Procedure

  1. Add 1 cup of Sucrose to a bottle
  2. Fill the bottle with distilled water until it reaches a final volume of 472.5 mL
    • As the bottle is being filled, swirl the contents so that the sucrose can begin to dissolve
  3. Autoclave the solution