Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 6th July 1.1 Lab work 1.1.1 Preparation of LB solid and liquid stock 1.1.2 Bringing DNA closer 1.1.2.1 1.1.3 Biobrick characterization 1.1.3.1 1.1.4 Visualization 1.1.4.1 Screen PCF for GBlock Wednesday 6th July Lab work Preparation of LB solid and liquid stock By Léa, Naiane, Laetitia - 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain. Bringing DNA closer By Biobrick characterization By Visualization Screen PCF for GBlock By Caroline "Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of + 42 μL MgCl2 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) 19 μL of mage was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clnes for 2.2).
By Léa, Naiane, Laetitia
- 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain.
By
By Caroline
"Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of + 42 μL MgCl2 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.)
19 μL of mage was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clnes for 2.2).
