Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 23rd June 1.1 Lab work 1.1.1 Heat-shock competent cells 1.1.2 Interlab study 1.1.2.1 Transformation results 1.1.2.2 Transformed cell cultures Thursday 23rd June Lab work Heat-shock competent cells By Caroline and Lea The cells competency was checked by transforming cells as usual with two control plasmids: the Interlab study negative control pSB1C3 blue Interlab study Transformation results By Caroline, Lea and Marion Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely. Transformed cell cultures By Lea One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.
By Caroline and Lea
The cells competency was checked by transforming cells as usual with two control plasmids:
By Caroline, Lea and Marion
Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely.
By Lea
One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.
