Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 6th July 1.1 Lab work 1.1.1 Preparation of LB solid and liquid stock 1.1.2 Bringing DNA closer 1.1.2.1 1.1.3 Biobrick characterization 1.1.3.1 1.1.4 Visualization 1.1.4.1 Screen PCR for GBlock Wednesday 6th July Lab work Preparation of LB solid and liquid stock By Léa, Naiane, Laetitia - 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain. Bringing DNA closer By Biobrick characterization By Visualization Screen PCR for GBlock By Caroline Megamix were prepared for 34 tubes with 70μL of 10xTaq buffer + 70 μL of dNTP 10mM + 42 μL MgCl2 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) 19 μL of megamix was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clones for 2.2).
By Léa, Naiane, Laetitia
- 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain.
By
By Caroline
Megamix were prepared for 34 tubes with 70μL of 10xTaq buffer + 70 μL of dNTP 10mM + 42 μL MgCl2 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.)
19 μL of megamix was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clones for 2.2).
