Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 6th July 1.1 Lab work 1.1.1 Preparation of LB solid and liquid stock 1.1.2 Biobrick characterization 1.1.2.1 1.1.3 Visualization 1.1.3.1 gBlocks PCR screening 1.1.3.2 pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks Wednesday 6th July Lab work Preparation of LB solid and liquid stock By Léa, Naiane, Laetitia - 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain. Biobrick characterization By Visualization gBlocks PCR screening By Caroline A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF. 1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007. pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks By Léa, Caroline and Laetitia The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.
By Léa, Naiane, Laetitia
- 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain.
By
By Caroline
A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF.
1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007.
By Léa, Caroline and Laetitia
The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.
