Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Monday 18th July 1.1 Lab work 1.1.1 Preparation of LB solid and liquid stock 1.1.2 Getting DNA closer 1.1.2.1 Electrophoresis of PCR products DS_SPcasN, DS_TDcasN, pZA21 and pZA31 1.1.3 Biobrick characterization 1.1.3.1 LB culture of electro-transformed BL21 cells (Cf) 1.1.4 Visualization 1.1.4.1 Electrophoresis of PCR products pPS16_004 Monday 18th July Lab work Preparation of LB solid and liquid stock By Caroline and Terrence - 1L of LB liquid : 20g/L powder LB + 1L water μQ - 500mL of LB solid : 500mL of LB liquid + 7.5g/L of Agar The all was put in the autoclave for sterilization. Getting DNA closer Electrophoresis of PCR products DS_SPcasN, DS_TDcasN, pZA21 and pZA31 By Caroline PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel. Biobrick characterization LB culture of electro-transformed BL21 cells (Cf) By Mathilde Each transformation : pclTAA + K1372001, pcl_TAG + K1372001 and pcl_Tq + K1372001 was added to 3mL of LB and one or two antibiotics. Three conditions were tested for each transformation : 15µL of Chloramphenicol (30µg/mL) 5 µL of Streptomycin (50µg/mL) 15µL of Chloramphenicol (30µg/mL) + 5 µL of Streptomycin (50µg/mL) Cultures were put in incubation at 37°c, 180 rpm. Visualization Electrophoresis of PCR products pPS16_004 By Caroline PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel
By Caroline and Terrence
- 1L of LB liquid : 20g/L powder LB + 1L water μQ - 500mL of LB solid : 500mL of LB liquid + 7.5g/L of Agar The all was put in the autoclave for sterilization.
By Caroline
PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel.
By Mathilde
Each transformation : pclTAA + K1372001, pcl_TAG + K1372001 and pcl_Tq + K1372001 was added to 3mL of LB and one or two antibiotics.
Three conditions were tested for each transformation :
Cultures were put in incubation at 37°c, 180 rpm.
PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel
