Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 19th July 1.1 Lab work 1.1.1 Biobrick characterization 1.1.2 Visualization 1.1.2.1 Ligation of gBlock 1.2, 2.2, 4.1 within pUC19 1.1.2.2 Transformation of DH5α with 1.2, 2.2, 4.1 Tuesday 19th July Lab work Biobrick characterization Visualization Ligation of gBlock 1.2, 2.2, 4.1 within pUC19 By Naiane & Charlène gBlock 1.2, 2.2, 4.1 were inserted in pUC19. Two controls were made : digested pUC19 with only water digested pUC19 without gBlock Transformation of DH5α with 1.2, 2.2, 4.1 By Naiane & Charlène HeatShock competent cells were transformed with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). Cells were plated on LB + Ampicillin + IPTG + Xgal : for each gBlock, one Petri dish with 50µL of cells and another with 150µL of cells for each control, 100µL of cells They were incubated overnight.
By Naiane & Charlène gBlock 1.2, 2.2, 4.1 were inserted in pUC19. Two controls were made :
By Naiane & Charlène HeatShock competent cells were transformed with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). Cells were plated on LB + Ampicillin + IPTG + Xgal :
They were incubated overnight.
