Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 19th July 1.1 Lab work 1.1.1 Biobrick characterization 1.1.1.1 Streak BL21 bacteria on LB plate 1.1.1.2 K1372001 pre-culture 1.1.2 Visualization 1.1.2.1 Ligation of gBlock 1.2, 2.2, 4.1 within pUC19 1.1.2.2 Transformation of DH5α with 1.2, 2.2, 4.1 Tuesday 19th July Lab work Biobrick characterization Streak BL21 bacteria on LB plate By Charlène BL21 from glycerol stock were streaked on LB and incubated at 37°C overnight. K1372001 pre-culture By Naiane One colony of K1372001 from a petri dish was Visualization Ligation of gBlock 1.2, 2.2, 4.1 within pUC19 By Naiane & Charlène gBlock 1.2, 2.2, 4.1 were inserted in pUC19. Two controls were made : digested pUC19 with only water digested pUC19 without gBlock Transformation of DH5α with 1.2, 2.2, 4.1 By Naiane & Charlène HeatShock competent cells were transformed with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). Cells were plated on LB + Ampicillin + IPTG + Xgal : for each gBlock, one Petri dish with 50µL of cells and another with 150µL of cells for each control, 100µL of cells They were incubated at 37°C overnight.
By Charlène
BL21 from glycerol stock were streaked on LB and incubated at 37°C overnight.
By Naiane
One colony of K1372001 from a petri dish was
By Naiane & Charlène
gBlock 1.2, 2.2, 4.1 were inserted in pUC19. Two controls were made :
HeatShock competent cells were transformed with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). Cells were plated on LB + Ampicillin + IPTG + Xgal :
They were incubated at 37°C overnight.
