Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 20th July 1.1 Lab work 1.1.1 1.1.2 Biobrick characterization 1.1.2.1 pclTAA, pclTAG and pclTq transformations 1.1.2.2 1.1.3 Visualization 1.1.3.1 1.1.3.2 1.1.3.3 1.1.4 Get DNA Closer 1.1.4.1 Electrophoresis of PCR products DS_SPcasN, DS_TDcasN and pZA21 1.1.4.2 pZA21 transformation Wednesday 20th July Lab work By Biobrick characterization pclTAA, pclTAG and pclTq transformations By Leatitia and Caroline In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pclTAA, pclTAG and pclTq. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C. By Visualization By By By Get DNA Closer Electrophoresis of PCR products DS_SPcasN, DS_TDcasN and pZA21 By Caroline PCR products obtained the 19/07/2016 were put to migrate for 30min in a 0,8% agarose gel. pZA21 transformation By Laetitia and Caroline In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pZA21. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Kanamycin (50µg/ml) and incubated overnight at 37°C.
By
By Leatitia and Caroline
In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pclTAA, pclTAG and pclTq. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C.
By Caroline
PCR products obtained the 19/07/2016 were put to migrate for 30min in a 0,8% agarose gel.
By Laetitia and Caroline
In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pZA21. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Kanamycin (50µg/ml) and incubated overnight at 37°C.
