Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 27th July 1.1 Lab work 1.1.1 Visualization 1.1.2 == 1.1.3 Biobrick Characterization 1.1.3.1 BL21 electrocompetent cells preparation for glycerol preparation Wednesday 27th July Lab work Visualization == By Biobrick Characterization BL21 electrocompetent cells preparation for glycerol preparation By Charlène We obtained transformed bacteria for every conditions made yesterday (except for controls). File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.
By
By Charlène
We obtained transformed bacteria for every conditions made yesterday (except for controls). File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg
We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.
