Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 27th July 1.1 Lab work 1.1.1 Visualization 1.1.2 == 1.1.3 Biobrick Characterization 1.1.3.1 BL21 electrocompetent cells preparation for glycerol preparation Wednesday 27th July Lab work Visualization == By Biobrick Characterization BL21 electrocompetent cells preparation for glycerol preparation By Charlène We obtained transformed bacteria for every conditions made yesterday (except for controls). Colonies of BL21 transformed with pcl_TAA and K1372001 Colonies of BL21 transformed with pcl_TAG and K1372001 Colonies of BL21 transformed with pcl_Tq and K1372001 We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.
By
By Charlène
We obtained transformed bacteria for every conditions made yesterday (except for controls).
We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.
