Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 28th July 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009 Thursday 28th July Lab work Visualization Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009 By Laetitia PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. Thus, the PCR mix was done for 12 tubes following the usual protocol. Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. PCR was done with a Tm at 57°C. Each PCR Product was put to migrate on an agarose gel.
By Laetitia
PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. Thus, the PCR mix was done for 12 tubes following the usual protocol. Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. PCR was done with a Tm at 57°C. Each PCR Product was put to migrate on an agarose gel.
