Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 28th July 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009 1.1.2 Biobrick Characterization 1.1.2.1 BL21 electrocompetent cells in glycerol stock Thursday 28th July Lab work Visualization Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009 By Mathilde and Laetitia PCR was performed on 6 clones for each plasmid. Thus, the PCR mix was done for 24 tubes following the usual protocol. Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. PCR was done with a Tm at 57°C. Each PCR Product was put to migrate on an agarose gel. Biobrick Characterization BL21 electrocompetent cells in glycerol stock By Charlène 1 mL of each clone cultured yesterday were put in 500µL of glycerol 60%. They were conserved at -30°C.
By Mathilde and Laetitia
PCR was performed on 6 clones for each plasmid.
Thus, the PCR mix was done for 24 tubes following the usual protocol.
Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG.
PCR was done with a Tm at 57°C.
Each PCR Product was put to migrate on an agarose gel.
By Charlène
1 mL of each clone cultured yesterday were put in 500µL of glycerol 60%. They were conserved at -30°C.
