Team:Paris Saclay/Notebook/August/4

Thursday 4st August

Lab work

Visualization

DNA Extraction of plasmids containing g blocks 1.1, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP

By Caroline, Naiane, Mathilde and Laetitia


Extraction was done on:


Puc 19 containing Clone
1.1 2, 9
1.2 4, 7, 11
GFP 7
3.1 4
NM 7, 11
dCas9_TD 1
dCas9_SP 1


The extraction was performed using the kit. We followed this protocol.


Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

  • 700 uL of culture
  • 300 uL of glycerol (60%)