Team:Paris Saclay/Notebook/August/4

Thursday 4st August

Lab work

Visualization

DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-

By Caroline, Naiane, Mathilde and Laetitia


Extraction was done on:


Puc 19 containing Clone
pPS16_001 2, 9
pPS16_002 4, 7, 11
pPS16_009 7
pPS16_003 4
DS-NMcasN- 7, 11
DS-TDcasN- 1
DS-SPcasN- 1


The extraction was performed using the kit. We followed this protocol.

Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

  • 700 uL of culture
  • 300 uL of glycerol (60%)