Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 5st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 DNA Extraction of DS-TDcasN- and DS-SPcasN- 1.1.1.2 DNA Extraction of DS-NMcasN- and pPS_001 1.1.1.3 Q5 PCR on DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 Friday 5st August Lab work Visualization DNA Extraction of DS-TDcasN- and DS-SPcasN- By Laetitia The extraction was performed using the kit. We followed this protocol. The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation. Hence we used: -1 ml of resuspension buffer -1 ml lysis buffer -1 ml precipitation buffer The colum was used several times in order to recover the maximum of DNA DNA Extraction of DS-NMcasN- and pPS_001 By Mathilde The extraction was performed on clone 11 for NM and clone 9 for 1.1 using the kit. We followed this protocol. Q5 PCR on DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 By Caroline The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 70°C. The specific primers for each parts were using.
By Laetitia
The extraction was performed using the kit. We followed this protocol.
The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation.
Hence we used:
-1 ml of resuspension buffer
-1 ml lysis buffer
-1 ml precipitation buffer
The colum was used several times in order to recover the maximum of DNA
By Mathilde
The extraction was performed on clone 11 for NM and clone 9 for 1.1 using the kit. We followed this protocol.
By Caroline
The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 70°C. The specific primers for each parts were using.
