Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 5st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Q5 PCR on pPS16_003, pPS16_004, pPS16_006 and pPS16_007 1.1.1.2 PCR Clean-up with the NucleoSpin kit Friday 5st August Lab work Visualization Q5 PCR on pPS16_003, pPS16_004, pPS16_006 and pPS16_007 By Caroline The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 70°C. The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET. Only, PCR form pPS16_003 and pPS16_004 showed a positive result. PCR Clean-up with the NucleoSpin kit By Caroline The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual protocol. But, I forgot to diluted the Buffet NT3 with 100mL ethanol befor I began the purification and so any result was obtained on the 0.8% agarose gel. File:T--Paris_Saclay--160722_Visualisation_PCRpPS16_002_7.JPG
By Caroline
The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 70°C. The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET. Only, PCR form pPS16_003 and pPS16_004 showed a positive result.
The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual protocol. But, I forgot to diluted the Buffet NT3 with 100mL ethanol befor I began the purification and so any result was obtained on the 0.8% agarose gel.
File:T--Paris_Saclay--160722_Visualisation_PCRpPS16_002_7.JPG
